AFT1 antibody (AA 179-228)

Catalog No. ABIN6748547

The Rabbit Polyclonal anti-AFT1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect AFT1 in samples from Human, Rat, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Monkey, Xenopus laevis and Hamster.

Quick Overview for AFT1 antibody (AA 179-228) (ABIN6748547)

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Reactivity: Human, Rat, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Monkey, Xenopus laevis, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AFT1 Antibody

Binding Specificity: AA 179-228

Brand: IHC-plus™

Specificity: Human ATF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Rabbit, Xenopus (100%) Mouse, Bovine (92%) Zebrafish (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa179-228 of human ATF1 (P18846, NP_005162). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Marmoset, Rat, Hamster, Elephant, Panda, Dog, Rabbit, Horse, Opossum, Guinea pig, Platypus, Xenopus (100%), Mouse, Bovine (92%), Lizard (85%), Zebrafish (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AFT1

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Alternative Name: ATF1

Background: Name/Gene ID: ATF1
Family: Transcription factor

Synonyms: ATF1, ATF-1, EWS-ATF1, FUS/ATF-1, Protein TREB36, TREB36

Gene ID: 466

NCBI Accession: NP_005162

UniProt: P18846

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades