EHF antibody (AA 121-170)

Catalog No. ABIN6748571

The Rabbit Polyclonal anti-EHF antibody is suitable to detect EHF in samples from Human, Mouse, Rat, Dog, Cow and Rabbit. It has been validated for WB, IHC and IHC (p).

Quick Overview for EHF antibody (AA 121-170) (ABIN6748571)

Target: EHF (Ets Homologous Factor (EHF))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EHF Antibody

Binding Specificity: AA 121-170

Specificity: Mouse EHF

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat (100%) Dog, Bovine, Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa121-170 of mouse Ehf (O70273, NP_031940). Percent identity by BLAST analysis: Mouse, Rat (100%), Elephant (92%), Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Zebra finch, Platypus (85%), Human (78%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody. Antibody does not work for IHC with mouse samples.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EHF

Target: EHF (Ets Homologous Factor (EHF))

Alternative Name: ESE3 / EHF

Background: Name/Gene ID: EHF

Synonyms: EHF, ESE3, ESE3B, ESE-3, ESE3 transcription factor, Ets homologous factor, ESEJ, HEHF

Gene ID: 26298

NCBI Accession: NP_031940

UniProt: Q9NZC4