AFT1 antibody (C-Term)

Catalog No. ABIN6748575

This anti-AFT1 antibody is a Rabbit Polyclonal antibody detecting AFT1 in WB, IHC and IHC (p). Suitable for Human, Cow, Zebrafish (Danio rerio) and Monkey.

Quick Overview for AFT1 antibody (C-Term) (ABIN6748575)

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Reactivity: Human, Cow, Zebrafish (Danio rerio), Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AFT1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human ATF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Zebrafish (100%) Mouse, Rat, Dog, Rabbit (92%) Xenopus (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human ATF1 (P18846, NP_005162). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Trout, Stickleback, Zebrafish (100%), Galago, Mouse, Rat, Hamster, Elephant, Panda, Dog, Rabbit, Horse, Opossum, Guinea pig (92%), Orangutan, Zebra finch, Platypus (85%), Lizard, Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AFT1

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Alternative Name: ATF1

Background: Name/Gene ID: ATF1
Family: Transcription factor

Synonyms: ATF1, ATF-1, EWS-ATF1, FUS/ATF-1, Protein TREB36, TREB36

Gene ID: 466

NCBI Accession: NP_005162

UniProt: P18846

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades