UBE2L3 antibody (AA 35-84)

Catalog No. ABIN6748597

This Rabbit Polyclonal antibody specifically detects UBE2L3 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Chicken, Xenopus laevis, Pig, Bat, Monkey and Hamster.

Quick Overview for UBE2L3 antibody (AA 35-84) (ABIN6748597)

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Chicken, Xenopus laevis, Pig, Bat, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2L3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBE2L3 Antibody

Binding Specificity: AA 35-84

Brand: IHC-plus™

Specificity: Human UBE2L3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human UBE2L3 (Q5R5I4, NP_003338). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Armadillo, Platypus, Xenopus, Frog, Catfish, Salmon, Stickleback, Zebrafish (100%), Baboon, Drosophila (92%), Beetle (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UBE2L3

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Alternative Name: UBE2L3 / UBCH7

Background: Name/Gene ID: UBE2L3

Synonyms: UBE2L3, E2-F1, L-UBC, UBCH7, UBCE7, Ubiquitin carrier protein L3, UbcM4, Ubiquitin-protein ligase L3

Gene ID: 7332

NCBI Accession: NP_003338

UniProt: P68036