FRZB antibody (AA 216-265)

Catalog No. ABIN6748620

The Rabbit Polyclonal anti-FRZB antibody has been validated for WB, IHC and IHC (p). It is suitable to detect FRZB in samples from Human, Mouse, Rat, Horse, Dog, Bat, Monkey and Hamster.

Quick Overview for FRZB antibody (AA 216-265) (ABIN6748620)

Target: FRZB (Frizzled-Related Protein (FRZB))

Reactivity: Human, Mouse, Rat, Horse, Dog, Bat, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FRZB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FRZB Antibody

Binding Specificity: AA 216-265

Brand: IHC-plus™

Specificity: Human FRZB

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog (100%) Pig, Chicken, Zebrafish (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa216-265 of human FRZB (Q92765, NP_001454). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Horse, Opossum (100%), Elephant, Bovine, Pig, Guinea pig, Turkey, Chicken, Zebrafish (92%), Zebra finch, Platypus, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FRZB

Target: FRZB (Frizzled-Related Protein (FRZB))

Alternative Name: SFRP-3 / FRZB

Background: Name/Gene ID: FRZB
Subfamily: Frizzled

Synonyms: FRZB, FRZB-1, FIZ, FRE, FRITZ, Fritz and frizzled, Frizzled homolog-related, FRP-3, FRZB-PEN, HFIZ, FRP, FZRB, SFRP-3, SRFP3, OS1, Frezzled, Frizzled related protein 3, Frizzled-related protein, Frizzled-related protein 1, FRZB1, SFRP3

Gene ID: 2487

NCBI Accession: NP_001454

UniProt: Q92765

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation