Reticulon 4 antibody (AA 251-357) (HRP)

Catalog No. ABIN674868

This anti-Reticulon 4 antibody is a Rabbit Polyclonal antibody detecting Reticulon 4 in WB, ELISA, IHC (p) and IHC (fro). Suitable for Human and Mouse.

Quick Overview for Reticulon 4 antibody (AA 251-357) (HRP) (ABIN674868)

Target: Reticulon 4 (RTN4)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Reticulon 4 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Reticulon 4 Antibody (HRP)

Binding Specificity: AA 251-357

Specificity: Based on the location of the target sequence this antibody will recognize all isoforms(Nogo-A, Nogo-B, and Nogo-C).

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Mouse Reticulon 4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Reticulon 4

Target: Reticulon 4 (RTN4)

Alternative Name: Reticulon 4

Background:

Synonyms: Reticulon-4, Rtn4, Neurite outgrowth inhibitor, Nogo protein, Foocen, Neuroendocrine-specific protein, NSP, euroendocrine-specific protein C homolog, RTN-x, Reticulon-5, RTN 4A, Nogo-A, RTN-xL, RTN 4B, ASY, Nogo-B, RTN-xS, Foocen-M, RTN 4C, Nogo-C, Foocen-S, RTN4-B2, Rtn-T

Background: Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The reticulon protein is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Reticulon 4 regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. It regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. May inhibit BACE1 activity and amyloid precursor protein processing.

Gene ID: 68585

UniProt: Q99P72

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome