GPR177/WLS antibody (AA 53-102)

Catalog No. ABIN1948692

This anti-GPR177/WLS antibody is a Rabbit Polyclonal antibody detecting GPR177/WLS in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Pig and Monkey.

Quick Overview for GPR177/WLS antibody (AA 53-102) (ABIN1948692)

Target: GPR177/WLS (WLS) (Wntless Homolog (WLS))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR177/WLS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GPR177/WLS Antibody

Binding Specificity: AA 53-102

Brand: IHC-plus™

Specificity: Human WLS / GPR177

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa53-102 of human WLS (Q96IV8). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Marmoset, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig (100%), Galago, Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GPR177/WLS

Target: GPR177/WLS (WLS) (Wntless Homolog (WLS))

Alternative Name: WLS / GPR177

Background: Name/Gene ID: WLS

Synonyms: WLS, C1orf139, G protein-coupled receptor 177, Protein wntless homolog, MRP, Wntless homolog (Drosophila), EVI, GPR177

Gene ID: 79971

Pathways: WNT Signaling