UCP1 antibody (AA 101-200)

Catalog No. ABIN675413

The Rabbit Polyclonal anti-UCP1 antibody has been validated for WB, ELISA, IHC (p), FACS, IF (cc), IF (p) and IHC (fro). It is suitable to detect UCP1 in samples from Human, Mouse, Rat and Goat. There are 3+ publications available.

Quick Overview for UCP1 antibody (AA 101-200) (ABIN675413)

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Reactivity: Human, Mouse, Rat, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-UCP1 Antibody

Binding Specificity: AA 101-200

Cross-Reactivity: Goat, Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UCP-1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-UCP1 antibody (ABIN675413)

Hou, Shi, Cao, Xu, Hu, Wang: "Pig has no uncoupling protein 1." in: Biochemical and biophysical research communications, Vol. 487, Issue 4, pp. 795-800, (2017) (PubMed).

Sun, Zeng, Cao, Song, Zhang, Liu: "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." in: BioMed research international, Vol. 2015, pp. 919401, (2015) (PubMed).

Lu, Ji, Zhang, Zhang, Zhang, An, Liu, Zheng: "Resistance to obesity by repression of VEGF gene expression through induction of brown-like adipocyte differentiation." in: Endocrinology, Vol. 153, Issue 7, pp. 3123-32, (2012) (PubMed).

Target Details for UCP1

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: UCP-1

Background:

Synonyms: Ucp, Slc25a7, AI385626, Mitochondrial brown fat uncoupling protein 1, UCP 1, Solute carrier family 25 member 7, Thermogenin, Ucp1

Background: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.

Gene ID: 22227

UniProt: P12242

Pathways: Proton Transport, Brown Fat Cell Differentiation