SPDEF/PSE antibody (AA 182-231)

Catalog No. ABIN6754879

Product Details anti-SPDEF/PSE Antibody

Target: SPDEF/PSE (SPDEF) (Proximal Sequence Element 1 (SPDEF))

Binding Specificity: AA 182-231

Reactivity: Human, Cow, Rabbit, Dog, Guinea Pig, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPDEF/PSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SPDEF

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa182-231 of human SPDEF (O95238, NP_036523). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Rabbit, Pig, Guinea pig (100%), Galago, Mouse, Rat, Hamster, Bat, Horse, Platypus (92%), Elephant, Opossum, Chicken, Lizard (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SPDEF/PSE

Target: SPDEF/PSE (SPDEF) (Proximal Sequence Element 1 (SPDEF))

Alternative Name: PDEF / SPDEF (SPDEF Products)

Synonyms: PDEF antibody, RP11-375E1__A.3 antibody, bA375E1.3 antibody, pdef antibody, Pse antibody, SAM pointed domain containing ETS transcription factor antibody, SAM pointed domain containing ets transcription factor antibody, SPDEF antibody, Spdef antibody

Background: Name/Gene ID: SPDEF
Subfamily: ETS
Family: Transcription factor

Synonyms: SPDEF, BA375E1.3, PDEF, RP11-375E1__A.3, Prostate-derived Ets factor, Prostate-specific Ets, PSE

Gene ID: 25803

NCBI Accession: NP_036523