UBE2K antibody (AA 108-157)

Catalog No. ABIN6754906

The Rabbit Polyclonal anti-UBE2K antibody has been validated for WB, IHC and IHC (p). It is suitable to detect UBE2K in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Goat, Horse, Rabbit, Guinea Pig, Chicken, Xenopus laevis, Pig, Monkey, Hamster and Bat.

Quick Overview for UBE2K antibody (AA 108-157) (ABIN6754906)

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Goat, Horse, Rabbit, Guinea Pig, Chicken, Xenopus laevis, Pig, Monkey, Hamster, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2K antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBE2K Antibody

Binding Specificity: AA 108-157

Brand: IHC-plus™

Specificity: Human UBE2K / HIP2

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa108-157 of human UBE2K (P61086, NP_005330). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus, Salmon, Zebrafish (100%), Rice, Grape (92%), Drosophila, Beetle (85%), Arabidopsis (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UBE2K

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Alternative Name: UBE2K / LIG

Background: Name/Gene ID: UBE2K

Synonyms: UBE2K, E2-25K, E2(25K), HIP2, HYPG, LIG, UBC1, Ubiquitin-protein ligase, HIP-2, Ubiquitin carrier protein

Gene ID: 3093

NCBI Accession: NP_005330