ACTN2 antibody (N-Term)

Catalog No. ABIN6755306

The Rabbit Polyclonal anti-ACTN2 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect ACTN2 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Pig, Bat and Monkey.

Quick Overview for ACTN2 antibody (N-Term) (ABIN6755306)

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACTN2 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human ACTN2

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human ACTN2 (P35609, NP_001094). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Bat, Rabbit, Guinea pig (100%), Dog (92%), Elephant, Bovine, Horse, Pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ACTN2

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Alternative Name: ACTN2

Background: Name/Gene ID: ACTN2

Synonyms: ACTN2, Actinin, alpha 2, Alpha-actinin skeletal muscle, CMD1AA, F-actin cross-linking protein, Alpha-actinin-2

Gene ID: 88

NCBI Accession: NP_001094

Pathways: Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Negative Regulation of Transporter Activity