RAC1 antibody (C-Term)

Catalog No. ABIN2627478

The Rabbit Polyclonal anti-RAC1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect RAC1 in samples from Human, Mouse, Rat, Dog, Monkey, Zebrafish (Danio rerio), Sheep, Rabbit, Horse, Cow, Chicken, Guinea Pig, Xenopus laevis, Bat and Pig.

Quick Overview for RAC1 antibody (C-Term) (ABIN2627478)

Target: RAC1

Reactivity: Human, Mouse, Rat, Dog, Monkey, Zebrafish (Danio rerio), Sheep, Rabbit, Horse, Cow, Chicken, Guinea Pig, Xenopus laevis, Bat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RAC1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human RAC1

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human RAC1 (P60763, NP_008839). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Trout, Seabass, Catfish, Salmon, Smelt, Stickleback, Pike, Zebrafish (100%), Sea squirt, Drosophila, Slime mold, Nematode, Beetle (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RAC1

Target: RAC1

Background: Name/Gene ID: RAC1
Family: Ras GTPase superfamily IPR001806

Synonyms: RAC1, MIG5, p21-Rac1, Ras-like protein TC25, TC25, TC-25, Rac-1

Gene ID: 5879

NCBI Accession: NP_008839

Pathways: WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling