BCKDK antibody (AA 111-160)

Catalog No. ABIN6756587

The Rabbit Polyclonal anti-BCKDK antibody (ABIN6756587) specifically detects BCKDK in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow, Pig and Hamster samples.

Quick Overview for BCKDK antibody (AA 111-160) (ABIN6756587)

Target: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Reactivity: Human, Mouse, Rat, Cow, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCKDK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BCKDK Antibody

Binding Specificity: AA 111-160

Specificity: Human BCKDK

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa111-160 of human BCKDK (O14874, NP_005872). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Mouse, Rat, Hamster, Panda, Bovine, Pig (100%), Galago, Marmoset, Dog, Guinea pig (92%), Horse (91%), Monkey, Bat, Rabbit (85%), Platypus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BCKDK

Target: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Alternative Name: BCKDK

Background: Name/Gene ID: BCKDK
Subfamily: PDK/BCKDK
Family: Protein Kinase

Synonyms: BCKDK, BCKDKD, BCKDH kinase, BCKDHKIN, BCODC kinase, BCODH kinase, BCKA dehydrogenase kinase, BCKAD kinase, BCKD-kinase, BDK

Gene ID: 10295

NCBI Accession: NP_005872

Pathways: SARS-CoV-2 Protein Interactome