GRF2 antibody (AA 866-915)

Catalog No. ABIN6756592

This Rabbit Polyclonal antibody specifically detects GRF2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Dog, Monkey, Cow, Guinea Pig, Horse, Pig, Bat, Chicken and Xenopus laevis.

Quick Overview for GRF2 antibody (AA 866-915) (ABIN6756592)

Target: GRF2 (RAPGEF1) (Rap Guanine Nucleotide Exchange Factor (GEF) 1 (RAPGEF1))

Reactivity: Human, Mouse, Dog, Monkey, Cow, Guinea Pig, Horse, Pig, Bat, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRF2 Antibody

Binding Specificity: AA 866-915

Brand: IHC-plus™

Specificity: Human RAPGEF1

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa866-915 of human RAPGEF1 (Q13905, NP_005303). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback (100%), Rat (93%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GRF2

Target: GRF2 (RAPGEF1) (Rap Guanine Nucleotide Exchange Factor (GEF) 1 (RAPGEF1))

Alternative Name: RAPGEF1

Background: Name/Gene ID: RAPGEF1

Synonyms: RAPGEF1, C3G, CRK SH3-binding GNRP, GRF2, Protein C3G

Gene ID: 2889

NCBI Accession: NP_005303

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor