PHGDH antibody (AA 254-303)

Catalog No. ABIN6756651

This anti-PHGDH antibody is a Rabbit Polyclonal antibody detecting PHGDH in WB, IHC, IHC (p). Suitable for Human, Mouse, Rat, Horse, Pig, Rabbit, Hamster, Bat.

Quick Overview for PHGDH antibody (AA 254-303) (ABIN6756651)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivity: Human, Mouse, Rat, Horse, Pig, Rabbit, Hamster, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHGDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PHGDH Antibody

Binding Specificity: AA 254-303

Brand: IHC-plus™

Specificity: Human PHGDH

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa254-303 of human PHGDH (O43175, NP_006614). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Panda, Bat, Rabbit, Horse, Pig (100%), Marmoset, Dog, Bovine, Guinea pig (92%), Xenopus (91%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: PHGDH

Background: Name/Gene ID: PHGDH

Synonyms: PHGDH, 3-PGDH, 3PGDH, PGDH, Phosphoglycerate dehydrogenase, PDG, PGAD, PGDH3

Gene ID: 26227

NCBI Accession: NP_006614

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect