PEPD antibody (AA 359-408)

Catalog No. ABIN6756656

Product Details anti-PEPD Antibody

Target: PEPD (Peptidase D (PEPD))

Binding Specificity: AA 359-408

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEPD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PEPD

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa359-408 of human PEPD (P12955, NP_000276). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Dog, Bat, Horse, Pig, Opossum, Guinea pig, Stickleback (100%), Monkey, Galago, Rabbit, Turkey, Chicken (92%), Bovine (91%), Zebrafish (85%), Elephant (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PEPD antibody (ABIN6756656)

Rešetar Maslov, Farkaš, Rubić, Kuleš, Beletić, Beer Ljubić, Šmit, Mrljak, Torti: "Serum Proteomic Profiles Reflect the Stages of Myxomatous Mitral Valve Disease in Dogs." in: International journal of molecular sciences, Vol. 24, Issue 8, (2023) (PubMed).

Target Details for PEPD

Target: PEPD (Peptidase D (PEPD))

Alternative Name: PEPD / PROLIDASE (PEPD Products)

Synonyms: MGC89151 antibody, DDBDRAFT_0190220 antibody, DDBDRAFT_0266378 antibody, DDB_0190220 antibody, DDB_0266378 antibody, PROLIDASE antibody, cb1000 antibody, fj78g11 antibody, wu:fj78g11 antibody, prolidase antibody, Pep-4 antibody, Pep4 antibody, peptidase D antibody, peptidase D L homeolog antibody, pepd antibody, pepD antibody, PEPD antibody, pepd.L antibody, Pepd antibody

Background: Name/Gene ID: PEPD
Subfamily: Metallopeptidase M24B
Family: Exopeptidase

Synonyms: PEPD, Aminoacyl-L-proline hydrolase, Imidodipeptidase, PRD, PROLIDASE, X-Pro dipeptidase, Xaa-Pro dipeptidase, Peptidase D, Proline dipeptidase

Gene ID: 5184

NCBI Accession: NP_000276