Calreticulin antibody (AA 301-350)

Catalog No. ABIN6756660

The Rabbit Polyclonal anti-Calreticulin antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Calreticulin in samples from Human, Mouse, Rat, Pig, Monkey, Guinea Pig, Dog, Cow, Rabbit, Hamster, Horse and Bat.

Quick Overview for Calreticulin antibody (AA 301-350) (ABIN6756660)

Target: Calreticulin (CALR)

Reactivity: Human, Mouse, Rat, Pig, Monkey, Guinea Pig, Dog, Cow, Rabbit, Hamster, Horse, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Calreticulin Antibody

Binding Specificity: AA 301-350

Brand: IHC-plus™

Specificity: Human CALR / Calreticulin

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa301-350 of human CALR (P27797, NP_004334). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Platypus (92%), Opossum, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: CALR / Calreticulin

Background: Name/Gene ID: CALR

Synonyms: CALR, Calregulin, CC1qR, CRP55, CRT, CRTC, ERp60, Grp60, RO, SSA, Calreticulin, HACBP

Gene ID: 811

NCBI Accession: NP_004334

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response