BMI1 antibody (AA 1-202)

Catalog No. ABIN6757080

This anti-BMI1 antibody is a Mouse Monoclonal antibody detecting BMI1 in WB, IP and ICC. Suitable for Human, Mouse, Rat and Rabbit.

Quick Overview for BMI1 antibody (AA 1-202) (ABIN6757080)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-BMI1 Antibody

Binding Specificity: AA 1-202

Specificity: Recognizes mouse Bmi-1 (triplet), Mr 40-44kD. Species cross-reactivity: human, rat and rabbit.

Purification: Protein G purified

Immunogen: Recombinant Bmi-1 protein corresponding to aa1-202 of mouse Bmi-1.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IP, WB (0.2 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunoprecipitation: Reported to immunoprecipitate Bmi-1 from mouse embryo protein extracts. Immunocytochemistry: Reported to immunostain U2OS cells fixed with 2 % formaldehyde. Positive control: USOS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine, 0.15 M sodium chloride,  pH 7.4, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1 / PCGF4

Background: Name/Gene ID: BMI1

Synonyms: BMI1, BMI-1, Flvi-2/bmi-1, FLVI2/BMI1, PCGF4, Polycomb group protein Bmi1, Polycomb complex protein BMI-1, Dna-binding protein bmi-1, Polycomb group ring finger 4, RING finger protein 51, RNF51

Gene ID: 648

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy