ZAP70 antibody (AA 326-341)

Catalog No. ABIN6757208

This anti-ZAP70 antibody is a Mouse Monoclonal antibody detecting ZAP70 in WB, ELISA and IP. Suitable for Human.

Quick Overview for ZAP70 antibody (AA 326-341) (ABIN6757208)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-ZAP70 Antibody

Binding Specificity: AA 326-341

Specificity: Recognizes ZAP-70 protein tyrosine kinase (PTK) and does not exhibit any cross-reactivity with the related Syk PTK Species cross-reactivity: Human, mouse.

Purification: Immunoaffinity purified

Immunogen: Peptide derived from a.a. 326-341 from the central portion of the human ZAP-70 protein sequence just upstream of the protein kinase domain. Shares 87.5% homology with the mouse ZAP-70 peptide.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IP, WB (1 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. ELISA: 0.1-1.0 μg/mL. Western Blot: 1 μg/mL. Immunoprecipitation: 5 μg. Positive control: Human Jurkat cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: Name/Gene ID: ZAP70
Subfamily: Syk/ZAP70
Family: Protein Kinase

Synonyms: ZAP70, 70 kDa zeta-associated protein, SRK, STCD, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, TZK, ZAP-70, Zeta-associated protein 70

Gene ID: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway