P-Cadherin antibody

Catalog No. ABIN6757209

The Mouse Monoclonal anti-P-Cadherin antibody has been validated for WB, IHC, ELISA, IF, IP, IHC (p) and Func. It is suitable to detect P-Cadherin in samples from Human.

Quick Overview for P-Cadherin antibody (ABIN6757209)

Target: P-Cadherin (CDH3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This P-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Product Details anti-P-Cadherin Antibody

Specificity: Recognizes human P cadherin.

Purification: Ion exchange chromatography

Immunogen: Epidermoid carcinoma cell line A431.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.5 μg/mL), Func, IF (2 - 5 μg/mL), IHC, IHC-P (2 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Also suitable as an inhibitor of P-cadherin dependent cell-cell contact. Immunofluorescence: 2-5 μg/mL. ELISA: 0.5 μg/mL using immobilized A431 cells. Western Blot (Native): 0.5-2 μg/mL. Immunohistochemistry (FFPE): 2 μg/mL, Requires heat induced epitope retrieval with citrate buffer,  pH 6.0, prior to staining. Inhibition of P-cadherin-dependent cell-cell contact: 100 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: TBS, 1 % BSA, 40 % glycerol

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for P-Cadherin

Target: P-Cadherin (CDH3)

Alternative Name: CDH3 / P-Cadherin

Background: Name/Gene ID: CDH3
Family: Cadherin

Synonyms: CDH3, Cadherin-3, HJMD, P-cadherin, CDHP, PCAD, Placental cadherin

Gene ID: 1001

UniProt: P22223