LRP5 antibody (C-Term)

Catalog No. ABIN6757408

This Rabbit Polyclonal antibody specifically detects LRP5 in WB and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for LRP5 antibody (C-Term) (ABIN6757408)

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-LRP5 Antibody

Binding Specificity: C-Term

Specificity: Recognizes human LRP5 protein. Western Blot tests using negative control mock pSEC-tag-transfected 293T cell lysates did not yield the target band. Species cross-reactivity: Mouse.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of the human LRP5 protein. This intracellular region is identical to the corresponding mouse and rat LRP5 amino acid sequences.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (2 - 3 μg/mL), WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence and Western Blot. Immunofluorescence: 2-3 μg/mL, Note: For optimal results in immunofluorescence assays, cells should be fixed in 4 % paraformaldehyde/0.5 % Triton X-100/PBS for 10 minutes, permeabilized with 0.5 % Triton X-100/PBS for 5 minutes, and blocked with 5 % BSA/PBS for 30 minutes. Western Blot: 1-3 μg/mL, Single band at ~200kD. Positive control: Mouse LRP5-pSEC-tag-transfected 293T cells, MCF-7 human breast adenocarcinoma, T47D human breast ductal carcinoma, and NIH-3T3 mouse embryo fibroblast lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for LRP5

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Alternative Name: LRP5

Background: Name/Gene ID: LRP5

Synonyms: LRP5, EVR1, Exudative vitreoretinopathy 1, LRP7, OPTA1, OPPG, OPS, LR3, LRP-5, VBCH2, BMND1, EVR4

Gene ID: 4041

UniProt: O75197

Pathways: WNT Signaling, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation