FZD3 antibody (Cysteine-Rich Domain, Extracellular)

Catalog No. ABIN6757417

This Goat Polyclonal antibody specifically detects FZD3 in WB, IHC and ICC. It exhibits reactivity toward Mouse.

Quick Overview for FZD3 antibody (Cysteine-Rich Domain, Extracellular) (ABIN6757417)

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-FZD3 Antibody

Binding Specificity: Cysteine-Rich Domain, Extracellular

Specificity: Recognizes mouse Frizzled-3 extracellular Cysteine-Rich Domain (CRD). Cross-reactivity: <1 % with rmFrizzled-1, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7 and rmFrizzled-8.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rmFrizzled-3 extracellular Cysteine-Rich Domain (CRD).

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC (5 - 15 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Direct ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Direct ELISA: 0.5-1 μg/mL. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-3. The detection limit is ~2 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 5-15 μg/mL in fixed cells or tissues.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FZD3

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Alternative Name: FZD3 / Frizzled 3

Background: Name/Gene ID: FZD3
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3

Gene ID: 7976

UniProt: Q9NPG1

Pathways: WNT Signaling, Tube Formation