COL6 antibody

Catalog No. ABIN6757442

The Rabbit Polyclonal anti-COL6 antibody is suitable to detect COL6 in samples from Human. It has been validated for WB, IHC, ELISA, IP and IHC (fro).

Quick Overview for COL6 antibody (ABIN6757442)

Target: COL6 (Collagen, Type VI (COL6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-COL6 Antibody

Specificity: Reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Purification: Immunoaffinity purified

Immunogen: Collagen Type VI from human and bovine placenta.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:4000), IHC, IHC-Fr, IP (1:50), WB (1:250 - 1:2500)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. ELISA: 1:2000-1:4000. 1 μg of Collagen VI was used in a standard sandwich ELISA with Goat anti-Rabbit IgG [H&L] (HRP conjugated) and (ABTS as a substrate for 30 minutes at room temperature. Western Blot: 1:250-1:2500. Immunoprecipitation: 1:50. Immunohistochemistry (Frozen tissue sections): 1:25-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA,  pH 8.0, 0.01 % sodium azide, before the addition of glycerol to 40 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for COL6

Target: COL6 (Collagen, Type VI (COL6))

Alternative Name: Collagen VI