His Tag antibody (C-Term)
This Rabbit Polyclonal antibody specifically detects His Tag in WB and ELISA. It exhibits reactivity toward .
Quick Overview for His Tag antibody (C-Term) (ABIN344299)
Target
See all His Tag Antibodies
His Tag
Reactivity
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Host
Rabbit
Clonality
Polyclonal
Conjugate
This His Tag antibody is un-conjugated
Application
Western Blotting (WB), ELISA
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Binding Specificity
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C-Term
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Sequence
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HHHHHH
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Specificity
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Reacts with over-expressed fusion proteins containing either amino- or carboxy-terminal His epitope tags.
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Purification
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Immunoaffinity purified
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Immunogen
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His epitope tag peptide HHHHHH conjugated to KLH.
Type of Immunogen: Synthetic peptide
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Isotype
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IgG
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Application Notes
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Approved: ELISA (0.05 - 0.2 μg/mL), WB (0.1 - 1 μg/mL)
Usage: Suitable for use in ELISA and Western Blot. ELISA: 0.05-0.2 μg/mL. Western Blot: 0.1-1.0 μg/mL.
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Restrictions
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For Research Use only
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Format
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Liquid
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Concentration
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Lot specific
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Buffer
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PBS, pH 7.4, 0.02 % sodium azide.
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Preservative
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Sodium azide
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Precaution of Use
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This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Target
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His Tag
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Target Type
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Tag
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