Sialylated Mucin-Type-O-Glycans antibody
Reactivity: Human
IHC (p), ELISA, IHC, ICC
Host: Mouse
Monoclonal
FW17
unconjugated
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- Target
- Sialylated Mucin-Type-O-Glycans
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Specificity
- Sialylated Mucin-Type-O-Glycans.
- Purification
- Purified
- Immunogen
- Purified mucus-glycoproteins from human amniotic fluid.
- Clone
- FW17
- Isotype
- IgM
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-
- Application Notes
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Approved: ELISA, ICC, IHC, IHC-P
Usage: Tumor and normal tissues were first fixed in 6 % neutral phosphate-buffered formalin and dehydrated in ethanol by standard histological techniques. After embedding all tissues in paraffin, serial microtome tissue sections (5 μm) were used for comparative immunohistological studies. Reactivity of hybridoma tissue culture supernatants was determined using a four-step immunoperoxidase technique. After deparaffinization in xylene, rehydration through graded ethanol, and blocking endogenous peroxidase activity with 2 % H202 in absolute methanol for 30 min, the sections were incubated as follows: (1) normal swine serum (5 % in PBS), (2) MAb in tissue culture supernatant or mouse ascites fluid diluted to 1/100 in PBS, (3) rabbit anti-mouse immunoglobulin (2 % in PBS), (4) swine anti-rabbit immunoglobulin (2 % in PBS), (5) rabbit peroxidase anti-peroxidase (PAP) complex. The slides were treated with 0.2 mg/mL - ' 3-amino-9-ethylcarbazole in 0.02 M sodium acetate buffer containing 0.01 % H202, then counterstained with hematoxylin, and mounted in glycerol jelly. All incubation steps were performed in a moist chamber at 21 °C for 30 min. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.4
- Storage
- 4 °C,-20 °C
- Storage Comment
- Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
-
- Target
- Sialylated Mucin-Type-O-Glycans
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