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EGF antibody (AA 31-53) (HRP)

The Rabbit Polyclonal anti-EGF antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect EGF in samples from Mouse.
Catalog No. ABIN676637

Quick Overview for EGF antibody (AA 31-53) (HRP) (ABIN676637)

Target

See all EGF Antibodies
EGF (Epidermal Growth Factor (EGF))

Reactivity

  • 164
  • 90
  • 59
  • 18
  • 15
  • 8
  • 5
  • 1
  • 1
Mouse

Host

  • 232
  • 54
  • 10
  • 2
  • 2
  • 1
Rabbit

Clonality

  • 234
  • 66
Polyclonal

Conjugate

  • 157
  • 36
  • 21
  • 12
  • 8
  • 6
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
This EGF antibody is conjugated to HRP

Application

  • 238
  • 110
  • 85
  • 70
  • 59
  • 53
  • 53
  • 27
  • 26
  • 17
  • 14
  • 13
  • 10
  • 8
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Binding Specificity

    • 44
    • 16
    • 14
    • 10
    • 8
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 31-53

    Cross-Reactivity

    Mouse

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic peptide derived from mouse EGF

    Isotype

    IgG
  • Application Notes

    WB 1:300-5000
    IHC-P 1:200-400
    IHC-F 1:100-500

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Handling Advice

    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    EGF (Epidermal Growth Factor (EGF))

    Alternative Name

    EGF

    Background

    Synonyms: AI790464, Pro-epidermal growth factor, EGF

    Background: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).

    Gene ID

    13645

    UniProt

    P01132

    Pathways

    NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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