Ret Proto-Oncogene antibody (AA 29-635)

Catalog No. ABIN6769310

This anti-Ret Proto-Oncogene antibody is a Mouse Monoclonal antibody detecting Ret Proto-Oncogene in WB, ELISA and FACS. Suitable for Human and Mouse.

Quick Overview for Ret Proto-Oncogene antibody (AA 29-635) (ABIN6769310)

Target: Ret Proto-Oncogene (RET)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ret Proto-Oncogene antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Ret Proto-Oncogene Antibody

Binding Specificity: AA 29-635

Specificity: Recognizes human Ret. Species cross-reactivity: Mouse.

Purification: Protein G purified

Immunogen: Recombinant protein corresponding to aa29-635 from human Ret/Fc Chimera expressed in Sf21 cells (P07949).

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow Cytometry, Western Blot and Direct ELISA. Flow Cytometry: 50 μg/mL, For intracellular staining to detect human Ret, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis. Western Blot: 1-2 μg/mL, Detection limit for rhRet is ~50 ng/lane under non-reducing and reducing conditions. Use chemiluminescence to increase sensitivity. Direct ELISA: 0.5-1.0 μg/mL, The detection limit for rhRet and rmRet is ~6 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile PBS to a concentration of 0.5 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

Target Details for Ret Proto-Oncogene

Target: Ret Proto-Oncogene (RET)

Alternative Name: RET

Background: Name/Gene ID: RET
Subfamily: Cadherin
Family: Protein Kinase

Synonyms: RET, Cadherin family member 12, CDHF12, C-ret, Hirschsprung disease 1, HSCR1, Hydroxyaryl-protein kinase, Oncogene ret, Proto-oncogene c-Ret, RET-ELE1, RET51, Ret proto-oncogene, RET transforming sequence, RET43, Men2-ret, MEN2B, MTC1, Receptor tyrosine kinase, CDHR16, MEN2A, PTC, RET9

Gene ID: 5979

UniProt: P07949

Pathways: RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation