Cytokeratin 18 antibody (AA 53-150)

Catalog No. ABIN676973

The Rabbit Polyclonal anti-Cytokeratin 18 antibody has been validated for WB, FACS, ELISA, IHC (p), IHC (fro), IF (cc) and IF (p). It is suitable to detect Cytokeratin 18 in samples from Human, Mouse, Rat and Pig. There are 4+ publications available.

Quick Overview for Cytokeratin 18 antibody (AA 53-150) (ABIN676973)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Cytokeratin 18 Antibody

Binding Specificity: AA 53-150

Cross-Reactivity: Human, Mouse, Pig, Rat

Predicted Reactivity: Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CK18

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Cytokeratin 18 antibody (ABIN676973)

Fagundes, Fernandes, Paraense, de Farias-Junior, Teixeira, Alves-Junior, Pinheiro, Crespo-López, Maia, Lima: "Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 7323627, (2017) (PubMed).

Liu, Feng, Dong, Yang, Li, Chen, Zhang, Wang, Zhou, Zhao: "Administration of BMSCs with muscone in rats with gentamicin-induced AKI improves their therapeutic efficacy." in: PLoS ONE, Vol. 9, Issue 5, pp. e97123, (2014) (PubMed).

Zeng, Zhang, Li, Yang, Chen, Liu: "A new method to isolate and culture rat kupffer cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e70832, (2013) (PubMed).

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: CK18

Background:

Synonyms: K18, CYK18, Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, KRT18, PIG46

Background: Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Gene ID: 3875

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis