IL-6 Receptor antibody

Catalog No. ABIN6774202

The Mouse Monoclonal anti-IL-6 Receptor antibody has been validated for WB, ELISA, FACS and Neut. It is suitable to detect IL-6 Receptor in samples from Human.

Quick Overview for IL-6 Receptor antibody (ABIN6774202)

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-6 Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut)

Clone: 17506

Product Details anti-IL-6 Receptor Antibody

Specificity: Recognizes human IL-6R.

Purification: Protein G purified

Immunogen: Sf 21-derived rhIL-6R.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (25 μg/mL), Neut, WB (1 - 2 μg/mL)

Usage: Suitable for use in Neutralization, Western Blot, ELISA and Flow Cytometry. Neutralization of Human IL6 R Bioactivity: ~0.1-0.2 μg/mL in the presence of 20 ng/mL of rhIL-6 R and 20 ng/mL of rhIL-6, using the M1 cell line. Western Blot: 1-2 μg/mL, The detection limit is ~100 ng/lane under non-reducing and reducing conditions. ELISA Capture: 2-8 μg/mL. Use biotinylated human IL-6 R detection antibody and recombinant human IL-6 R as the standard. Flow Cytometry: 25 μg/mL, 10 μL labels 1-2.5x10^5 U937 cells in a total reaction volume <200 μL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized, PBS, 5 % trehalose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IL-6 Receptor

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Alternative Name: IL6R / IL6 Receptor

Background: Name/Gene ID: IL6R
Family: Interleukin

Synonyms: IL6R, CD126, gp80, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL6Q, IL-6 receptor subunit alpha, IL-6R 1, IL-6R-1, Interleukin 6 receptor, Membrane glycoprotein 80, CD126 antigen, IL6RA, IL6RQ

Gene ID: 3570

UniProt: P08887

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints