IL-25 antibody

Catalog No. ABIN6774203

This anti-IL-25 antibody is a Rat Monoclonal antibody detecting IL-25 in ELISA and FACS. Suitable for Mouse.

Quick Overview for IL-25 antibody (ABIN6774203)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-25 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Product Details anti-IL-25 Antibody

Specificity: Recognizes mouse IL-17E. In sandwich ELISAs no cross-reactivity or interference was observed with rhIL-17E, rmIL-17, rmIL-17B, C, D, or F.

Purification: Protein G purified

Immunogen: E. coli-derived rmIL-17E.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Suitable for use in ELISA and Flow Cytometry. ELISA (Capture): 2-8 μg/m. Can be used as a capture reagent in a sandwich immunoassay in combination with a biotinylated, mouse IL-17E antibody and recombinant mouse IL-17E, as the standard. Flow Cytometry: For intracellular flow cytometry to detect IL-17E, cells must first be fixed and permeabilized with 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibody may be visualized by adding a secondary developing reagent such as anti-rat IgG conjugated to a fluorochrome.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 40-50 % glycerol, PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for IL-25

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: IL25 / IL17E

Background: Name/Gene ID: IL25
Family: Interleukin

Synonyms: IL25, Interleukin 17E, Interleukin 25, Interleukin-25, IL17E, IL-17E, IL-25, Interleukin-17E

Gene ID: 64806

UniProt: Q9H293