COL4a3 antibody (AA 1571-1670)

Catalog No. ABIN678128

The Rabbit Polyclonal anti-COL4a3 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect COL4a3 in samples from Mouse and Rat. There are 2+ publications available.

Quick Overview for COL4a3 antibody (AA 1571-1670) (ABIN678128)

Target: COL4a3 (COL4A3) (Collagen, Type IV, alpha 3 (COL4A3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL4a3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-COL4a3 Antibody

Binding Specificity: AA 1571-1670

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Tumstatin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-COL4a3 antibody (ABIN678128)

Sun, Liu, Pei, Yao, Ma, Mu, Wang, Zhang, Yang, Wang, Xue, Zhai, Carare, Qin, Yan: "The impairment of intramural periarterial drainage in brain after subarachnoid hemorrhage." in: Acta neuropathologica communications, Vol. 10, Issue 1, pp. 187, (2022) (PubMed).

Yasuda, Fukui, Okada, Yamawaki: "T3 peptide, a fragment of tumstatin, stimulates proliferation and migration of cardiac fibroblasts through activation of Akt signaling pathway." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 390, Issue 11, pp. 1135-1144, (2017) (PubMed).

Target Details for COL4a3

Target: COL4a3 (COL4A3) (Collagen, Type IV, alpha 3 (COL4A3))

Alternative Name: Tumstatin

Background:

Synonyms: Collagen alpha-3IV chain, Alpha 3 type IV collagen, Alpha3 type IV collagen, COL4A 3, COL4A3, Collagen IV alpha 3 polypeptide, Collagen type IV alpha 3 Goodpasture antigen, Collagen type IV alpha 3, Goodpasture antigen, OTTHUMP00000195044, Tumstatin, Col4a3, CO4A3_HUMAN.

Background: COL4A3 (Collagen, type IV, alpha 3) belongs to the type IV collagen family. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Type IV collagen is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity.

Gene ID: 1285

Pathways: Sensory Perception of Sound, Positive Regulation of Endopeptidase Activity