COL4a3 antibody (AA 1571-1670)

Catalog No. ABIN678128

Product Details anti-COL4a3 Antibody

Target: COL4a3 (COL4A3) (Collagen, Type IV, alpha 3 (COL4A3))

Binding Specificity: AA 1571-1670

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL4a3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Tumstatin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-COL4a3 antibody (ABIN678128)

Sun, Liu, Pei, Yao, Ma, Mu, Wang, Zhang, Yang, Wang, Xue, Zhai, Carare, Qin, Yan: "The impairment of intramural periarterial drainage in brain after subarachnoid hemorrhage." in: Acta neuropathologica communications, Vol. 10, Issue 1, pp. 187, (2022) (PubMed).

Yasuda, Fukui, Okada, Yamawaki: "T3 peptide, a fragment of tumstatin, stimulates proliferation and migration of cardiac fibroblasts through activation of Akt signaling pathway." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 390, Issue 11, pp. 1135-1144, (2017) (PubMed).

Target Details for COL4a3

Target: COL4a3 (COL4A3) (Collagen, Type IV, alpha 3 (COL4A3))

Alternative Name: Tumstatin (COL4A3 Products)

Synonyms: zTumstatin antibody, [a]3(IV) antibody, alpha3(IV) antibody, collagen type IV alpha 3 chain antibody, collagen, type IV, alpha 3 antibody, COL4A3 antibody, col4a3 antibody, Col4a3 antibody

Background:

Synonyms: Collagen alpha-3IV chain, Alpha 3 type IV collagen, Alpha3 type IV collagen, COL4A 3, COL4A3, Collagen IV alpha 3 polypeptide, Collagen type IV alpha 3 Goodpasture antigen, Collagen type IV alpha 3, Goodpasture antigen, OTTHUMP00000195044, Tumstatin, Col4a3, CO4A3_HUMAN.

Background: COL4A3 (Collagen, type IV, alpha 3) belongs to the type IV collagen family. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Type IV collagen is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity.

Gene ID: 1285

Pathways: Sensory Perception of Sound, Positive Regulation of Endopeptidase Activity