DKK1 antibody (AA 201-266)

Catalog No. ABIN678158

The Rabbit Polyclonal anti-DKK1 antibody has been validated for WB, ELISA, IHC (p), IF (p), IF (cc) and IHC (fro). It is suitable to detect DKK1 in samples from Human and Dog. There are 3+ publications available.

Quick Overview for DKK1 antibody (AA 201-266) (ABIN678158)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DKK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DKK1 Antibody

Binding Specificity: AA 201-266

Cross-Reactivity: Dog, Human

Predicted Reactivity: Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DKK1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-DKK1 antibody (ABIN678158)

Duan, Yan, Chen, Wu, Han, Guo, Qiao: "TET1 inhibits EMT of ovarian cancer cells through activating Wnt/β-catenin signaling inhibitors DKK1 and SFRP2." in: Gynecologic oncology, Vol. 147, Issue 2, pp. 408-417, (2017) (PubMed).

Lu, Duan, Zhang, Wu, Wang: "Expression of Wnt3a, Wnt10b, ?-catenin and DKK1 in periodontium during orthodontic tooth movement in rats." in: Acta odontologica Scandinavica, pp. 1-7, (2015) (PubMed).

Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).

Target Details for DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: DKK1

Background:

Synonyms: SK, DKK-1, Dickkopf-related protein 1, Dickkopf-1, hDkk-1, DKK1, UNQ492/PRO18

Background: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Gene ID: 22943

UniProt: O94907

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation