NQO1 antibody (AA 201-274)

Catalog No. ABIN678428

The Rabbit Polyclonal anti-NQO1 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect NQO1 in samples from Human, Mouse and Rat. There are 7+ publications available.

Quick Overview for NQO1 antibody (AA 201-274) (ABIN678428)

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NQO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NQO1 Antibody

Binding Specificity: AA 201-274

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Pig,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NQO1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NQO1 antibody (ABIN678428)

Zhang, Qu, Zheng, Zhang, Zhou, Cui, Mo, Li, Xu, Gao: "Nrf2 antioxidant pathway suppresses Numb-mediated epithelial-mesenchymal transition during pulmonary fibrosis." in: Cell death & disease, Vol. 9, Issue 2, pp. 83, (2019) (PubMed).

Zhang, Wang, Xie, Chen, Sinkemani, Yu, Wang, Mao, Wu: "Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence." in: Oncotarget, Vol. 9, Issue 2, pp. 2175-2192, (2018) (PubMed).

Shen, Wu, Wang, Zhao, Zhang, Li, Zhao: "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." in: International immunopharmacology, Vol. 46, pp. 16-22, (2017) (PubMed).

Tang, Gao, Mao, He, Liu, Cai, Lin, Wu: "Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways." in: Cell stress & chaperones, Vol. 21, Issue 2, pp. 239-49, (2016) (PubMed).

Wang, Peng, Wei, Wei, Wang, Ma, Yao, Fu, Zu: "Geraniin exerts cytoprotective effect against cellular oxidative stress by upregulation of Nrf2-mediated antioxidant enzyme expression via PI3K/AKT and ERK1/2 pathway." in: Biochimica et biophysica acta, Vol. 1850, Issue 9, pp. 1751-61, (2015) (PubMed).

Zhao, Shi, Li, Li, Zhao: "Silymarin attenuates paraquat-induced lung injury via Nrf2-mediated pathway in vivo and in vitro." in: Clinical and experimental pharmacology & physiology, (2015) (PubMed).

Target Details for NQO1

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Alternative Name: NQO1

Background:

Synonyms: DTD, QR1, DHQU, DIA4, NMOR1, NMORI, NAD(P)H dehydrogenase [quinone] 1, Azoreductase, DT-diaphorase, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, NQO1

Background: The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

Gene ID: 1728

UniProt: P15559