ARHGEF2 antibody (pSer886)

Catalog No. ABIN678563

The Rabbit Polyclonal anti-ARHGEF2 antibody is suitable to detect ARHGEF2 in samples from Human. It has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro).

Quick Overview for ARHGEF2 antibody (pSer886) (ABIN678563)

Target: ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ARHGEF2 Antibody

Binding Specificity: pSer886

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Arhgef2 around the phosphorylation site of Ser886

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ARHGEF2

Target: ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

Alternative Name: GEF H1

Background:

Synonyms: GEF H1phospho S886, Arhgef2Ser886, Lbcl1, Lfc, mKIAA0651, AA408978, ARHG2, ARHGEF 2, ARHGEF-2, ARHGEF2, GEF, GEF H1, GEF-H1, GEFH1, LFP40, MGC95068, P40, Prolerating cell nucleolar antigen p40, Protein GEF-H1, rho/rac guanine nucleotide exchange factor GEF 2, rho/rac guanine nucleotide exchange factor 2, rho/rac guanine nucleotide exchange factor.

Background: GEF-H1 is a Rho guanine nucleotide exchange factor that localizes to microtubules and regulates Rho activity in response to microtubule destabilization (loss of interaction between GEF-H1 and microtubules leads to activation of Rho). This protein has also been shown to localize to tight junctions and modulate polarized cell permeability.

Gene ID: 9181

Pathways: Negative Regulation of intrinsic apoptotic Signaling