CD80 antibody (AA 251-321) (HRP)

Catalog No. ABIN678692

The Rabbit Polyclonal anti-CD80 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect CD80 in samples from Human, Mouse and Rat.

Quick Overview for CD80 antibody (AA 251-321) (HRP) (ABIN678692)

Target: CD80

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD80 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD80 Antibody (HRP)

Binding Specificity: AA 251-321

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat CD80

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background:

Synonyms: B7-1, Activation B7 1 antigen, Activation B71 antigen, B lymphocyte activation antigen B7, B7, BB1, CD 80, CD28 antigen ligand 1 B7 1 antigen, CD28 antigen ligand 1 B71 antigen, CD28LG, CD28LG1, CD80 antigen, CD80 antigen precursor,CD80 Molecule, Costimulatory factor CD80, CTLA 4 counter receptor B7.1, CTLA-4 counter-receptor B7.1, LAB 7, LAB7, T lymphocyte activation antigen CD80, CD80_MOUSE.

Background: CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.

Gene ID: 25408

UniProt: G3V671

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints