Small Cajal Body-Specific RNA 22 (SCARNA22) antibody (HRP)

Catalog No. ABIN678977

The Rabbit Polyclonal anti- antibody has been validated for ELISA, IHC (fro) and IHC (p). It is suitable to detect in samples from Arabidopsis thaliana.

Quick Overview for Small Cajal Body-Specific RNA 22 (SCARNA22) antibody (HRP) (ABIN678977)

Target: Small Cajal Body-Specific RNA 22 (SCARNA22)

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Cross-Reactivity (Details): Arabidopsis thaliana,

Predicted Reactivity: Plant

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Arabidopsis thaliana ACA11

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Small Cajal Body-Specific RNA 22 (SCARNA22)

Alternative Name: Aca11

Background:

Synonyms: calcium-transporting ATPase, Putative calcium-transporting ATPase 11, plasma membrane-type, Ca2+-ATPase isoform 11, autoinhibited Ca2+-ATPase 11Arabidopsis thaliana, ACA11_ARATH.

Background: In plant cells, the vacuole functions as a major calcium store. Although a calmodulin-regulated Ca2+-ATPase (ACA4) is known to be present in prevacuolar compartments, the presence of an ACA-type Ca2+-ATPase in the mature vacuole of a plant cell has not been verified. Here we provide evidence that ACA11 localizes to the vacuole membrane. ACA11 tagged with GFP was expressed in stable transgenic plants, and visualized in root cells and protoplasts by confocal microscopy. A Ca2+-ATPase function for ACA11 was confirmed by complementation of yeast mutants.

Gene ID: 677770

UniProt: O22218