DGAT1 antibody (AA 251-350) (Cy3)

Catalog No. ABIN679571

The Rabbit Polyclonal anti-DGAT1 antibody has been validated for WB, IF (cc), IF (p). It is suitable to detect DGAT1 in samples from Human, Mouse, Rat, Dog.

Quick Overview for DGAT1 antibody (AA 251-350) (Cy3) (ABIN679571)

Target: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGAT1 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-DGAT1 Antibody (Cy3)

Binding Specificity: AA 251-350

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DGAT1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DGAT1

Target: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Alternative Name: Dgat1/Diglyceride Acyltransferase

Background:

Synonyms: ARAT, DGAT, ARGP1, DIAR7, Diacylglycerol O-acyltransferase 1, ACAT-related gene product 1, Acyl-CoA retinol O-fatty-acyltransferase, Retinol O-fatty-acyltransferase, Diglyceride acyltransferase, DGAT1, AGRP1

Background: Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.

Gene ID: 8694

UniProt: O75907

Pathways: Hormone Transport