CDC25A antibody (AA 451-524)

Catalog No. ABIN680263

This Rabbit Polyclonal antibody specifically detects CDC25A in WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for CDC25A antibody (AA 451-524) (ABIN680263)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CDC25A Antibody

Binding Specificity: AA 451-524

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: synthetic peptide from human cdc25A conjugated to KLH

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CDC25A antibody (ABIN680263)

Wei, Zhang, Ren, Zhang, Liu, Duan, Yu, Li, Peng, Zhou, Sun: "Endosulfan induces cell dysfunction through cycle arrest resulting from DNA damage and DNA damage response signaling pathways." in: The Science of the total environment, Vol. 589, pp. 97-106, (2017) (PubMed).

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: Cdc25A

Background:

Synonyms: Cdc 25a, CDC25A2, Cell division cycle 25 homolog A S. pombe, Cell division cycle 25A, Cell division cycle 25A isoform a, Cell division cycle 25A isoform bv, D9Ertd393ev, Dual specicity phosphatase CDC25A, EC 3.1.3.48, M phase inducer phosphatase 1, MGC115549.

Background: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Gene ID: 993

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase