SQSTM1 antibody (AA 51-150)

Catalog No. ABIN682153

This Rabbit Polyclonal antibody specifically detects SQSTM1 in WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Cow and has been mentioned in 5+ publications.

Quick Overview for SQSTM1 antibody (AA 51-150) (ABIN682153)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SQSTM1 Antibody

Binding Specificity: AA 51-150

Cross-Reactivity: Cow, Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SQSTM1/Ubiquitin-binding protein p62

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SQSTM1 antibody (ABIN682153)

Kuo, Weng, Chang, Huang, Lin, Chuang, Lin, Liou, Tai, Lin, Wang: "Altered mitochondrial dynamics and response to insulin in cybrid cells harboring a diabetes-susceptible mitochondrial DNA haplogroup." in: Free radical biology & medicine, Vol. 96, pp. 116-29, (2016) (PubMed).

Xia, Gao, Zhang, Wang, Zhao, Che, Ao, Yang, Wang, Lei: "Autophagy mediated by arginine depletion activation of the nutrient sensor GCN2 contributes to interferon-γ-induced malignant transformation of primary bovine mammary epithelial cells." in: Cell death discovery, Vol. 2, pp. 15065, (2016) (PubMed).

Cui, Luo, Li, Li, Wang: "Changes of intracellular Ca2+ in quercetin-induced autophagy progression." in: Acta biochimica et biophysica Sinica, Vol. 47, Issue 11, pp. 908-14, (2015) (PubMed).

Chen, Wei, Nie, Lin, Tian, Liu, Yu, Cheng, Yan, Wang, Liu, Deng, Lai, Zhou, Zhang, Lin, Chen: "?-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice." in: Brain research, Vol. 1552, pp. 41-54, (2014) (PubMed).

Wang, Gao, Yao, Xie: "Blocking autophagic flux enhances matrine-induced apoptosis in human hepatoma cells." in: International journal of molecular sciences, Vol. 14, Issue 12, pp. 23212-30, (2014) (PubMed).

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1

Background:

Synonyms: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, SQSTM1, ORCA

Background: Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.

Gene ID: 8878

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy