ASK1 antibody (pThr845)

Catalog No. ABIN683128

This Rabbit Polyclonal antibody specifically detects ASK1 in WB, ELISA, IHC (p) and FACS. It exhibits reactivity toward Human, Mouse, Rat and Cow and has been mentioned in 2+ publications.

Quick Overview for ASK1 antibody (pThr845) (ABIN683128)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-ASK1 Antibody

Binding Specificity: pThr845

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse ASK1 around the phosphorylation site of Thr845

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ASK1 antibody (ABIN683128)

Zhou, Zhang, Hu, Gu, Cai, Wu, Liu, Jiang, Kong, Zhang: "Inhibition of apoptosis signal-regulating kinase by paeoniflorin attenuates neuroinflammation and ameliorates neuropathic pain." in: Journal of neuroinflammation, Vol. 16, Issue 1, pp. 83, (2019) (PubMed).

Taniuchi, Furihata, Hanazaki, Iwasaki, Tanaka, Shimizu, Saito, Saibara: "Peroxiredoxin 1 Promotes Pancreatic Cancer Cell Invasion by Modulating p38 MAPK Activity." in: Pancreas, Vol. 44, Issue 2, pp. 331-40, (2015) (PubMed).

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: ASK1

Background:

Synonyms: ASK, ASK1, Mekk5, MAPKKK5, 742452D2Rik, Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, ASK-1, MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5, Map3k5

Background: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).

Gene ID: 26408

UniProt: O35099

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response