CEBPA antibody (pSer21)

Catalog No. ABIN683548

This Rabbit Polyclonal antibody specifically detects CEBPA in ELISA, IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CEBPA antibody (pSer21) (ABIN683548)

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPA antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CEBPA Antibody

Binding Specificity: pSer21

Predicted Reactivity: Human,Mouse,Rat,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CDBP alpha around the phosphorylation site of Ser21

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CEBPA

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Alternative Name: CEBP alpha

Background:

Synonyms: Apoptotic cysteine protease, Apoptotic protease Mch 5, C/EBP alpha, CAP4, Caspase 8 precursor, CCAAT Enhancer Binding Protein alpha, CEBP, CEBP A, CEBP alpha, CEBPA, FADD homologous ICE/CED 3 like protease, FADD like ICE, FLICE, ICE like apoptotic protease 5, ICE8, MACH, MCH5, MORT1 associated CED 3 homolog, CEBPA_HUMAN.

Background: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Mutations in this gene are frequently found in acute myeloid leukemia (AML).

Gene ID: 1050

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation