CBL antibody (pTyr731) (HRP)

Catalog No. ABIN683977

This Rabbit Polyclonal antibody specifically detects CBL in IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CBL antibody (pTyr731) (HRP) (ABIN683977)

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBL antibody is conjugated to HRP

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CBL Antibody (HRP)

Binding Specificity: pTyr731

Purpose: phospho-CBL (Tyr731) Polyclonal Antibody, HRP Conjugated

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CBL2 around the phosphorylation site of Tyr731

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CBL

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Alternative Name: CBL2

Background:

Synonyms: C CBL Tyr731, CBL2 Tyr731, CBL2 Tyr731, p-CBL2 Tyr731, p-CBL2 Y731, C CBL, Cas-Br-M murine ecotropic retroviral transforming sequence, Casitas B lineage lymphoma proto oncogene, CBL 2,E3 ubiquitin protein ligase CBL, Oncogene CBL2, Proto oncogene c CBL, RGD1561386, RING finger protein 55, RNF55v Signal transduction protein CBL, 4732447J05Rik, CBL_HUMAN.

Background: The proto oncogene c CBL was initially identified as the cellular homologue of v CBL oncogene that induces pre B cell lymphomas and myeloid leukemias in mice. In more recent studies CBL has been shown to be a negative regulator of tyrosine kinase signaling. The ubiquitin ligase activity of CBL leads to the degradation of tyrosine kinases, thus attenuating the signal of receptors. Targets of CBL include activated protein tyrosine kinases belonging to the Src and Syk/Zap 70 families. An additional mechanism to attenuate receptor signaling is thought to be achieved by CBL?s interaction with downstream targets of tyrosine kinases, such as PI 3K and Vav.

Gene ID: 867

Pathways: TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals