CDC25A antibody (pThr507) (HRP)

Catalog No. ABIN684037

This Rabbit Polyclonal antibody specifically detects CDC25A in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for CDC25A antibody (pThr507) (HRP) (ABIN684037)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CDC25A Antibody (HRP)

Binding Specificity: pThr507

Specificity: This phosphorylation site is homologous to Thr497 in Mouse and Thr508 in Rat.

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human cdc25A around the phosphorylation site of Thr507 [SR(p-T)WA]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: cdc25A

Background:

Synonyms: CDC25A2, M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A

Background: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Gene ID: 993

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase