CDC25A antibody (pSer76)

Catalog No. ABIN684043

This Rabbit Polyclonal antibody specifically detects CDC25A in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for CDC25A antibody (pSer76) (ABIN684043)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CDC25A Antibody

Binding Specificity: pSer76

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human cdc25A around the phosphorylation site of Ser76 [MG(p-S)SE]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: cdc25A

Background:

Synonyms: cdc25ASer76, Cdc 25a, CDC25A2, Cell division cycle 25 homolog A S. pombe, Cell division cycle 25A, Cell division cycle 25A isoform a, Cell division cycle 25A isoform bv D9Ertd393ev Dual specicity phosphatase CDC25A, EC 3.1.3.48, M phase inducer phosphatase 1, MGC115549,

Background: Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

Gene ID: 993

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase