CDC25A antibody (pSer178) (HRP)

Catalog No. ABIN684067

This Rabbit Polyclonal antibody specifically detects CDC25A in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CDC25A antibody (pSer178) (HRP) (ABIN684067)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CDC25A Antibody (HRP)

Binding Specificity: pSer178

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human cdc25A around the phosphorylation site of Ser178 [QN(p-S)AP]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: cdc25A

Background:

Synonyms: Cdc 25a, CDC25A2, Cell division cycle 25 homolog A S. pombe, Cell division cycle 25A, Cell division cycle 25A isoform a, Cell division cycle 25A isoform bv D9Ertd393ev Dual specicity phosphatase CDC25A, EC 3.1.3.48, M phase inducer phosphatase 1, MGC115549.

Background: Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

Gene ID: 993

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase