Clusterin antibody (AA 331-449)

Catalog No. ABIN685342

This Rabbit Polyclonal antibody specifically detects Clusterin in ELISA, IHC (p), FACS, IHC (fro), IF (cc) and IF (p). It exhibits reactivity toward Human, Rat and Mouse and has been mentioned in 2+ publications.

Quick Overview for Clusterin antibody (AA 331-449) (ABIN685342)

Target: Clusterin (CLU)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Clusterin Antibody

Binding Specificity: AA 331-449

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Apolipoprotein J

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Clusterin antibody (ABIN685342)

Keck, Androsova, Gualtieri, Walker, von Rüden, Russmann, Deeg, Hauck, Krause, Potschka: "A systems level analysis of epileptogenesis-associated proteome alterations." in: Neurobiology of disease, Vol. 105, pp. 164-178, (2017) (PubMed).

Yu, Chen, Wang, Zhou, Luo, Xu, Shen, Yin, Tao, Xiao, Xiao, Lü, Wang: "Time-dependent decrease of clusterin as a potential cerebrospinal fluid biomarker for drug-resistant epilepsy." in: Journal of molecular neuroscience : MN, Vol. 54, Issue 1, pp. 1-9, (2014) (PubMed).

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: Apolipoprotein J

Background:

Synonyms: CLI, AAG4, APOJ, CLU1, CLU2, KUB1, SGP2, APO-J, SGP-2, SP-4, TRPM2, TRPM-2, NA1/NA2, Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Complement-associated protein SP-4,4, Ku7-binding protein 1, Testosterone-repressed prostate message 2, CLU

Background: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC7. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

Gene ID: 1191

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling