Iba1 antibody (AA 51-147)

Catalog No. ABIN685477

This independently validated Rabbit Polyclonal antibody specifically detects Iba1 in WB, ELISA, IHC (p), FACS, IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human, Rat and Mouse and has been mentioned in 7+ publications.

Quick Overview for Iba1 antibody (AA 51-147) (ABIN685477)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Iba1 Antibody

Binding Specificity: AA 51-147

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human AIF1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Independent Validation (IF)

Validation #029613 (Immunofluorescence)

by: Reveal Biosciences

No.: #029613

Date: 02/21/2014

Lot Number: 980993W

Method validated: Immunofluorescence

Positive Control: Mouse spleen

Negative Control: Mouse duodenum

Notes: Strong signal was observed in the positive control tissue, while no signal was observed in the negative control tissue.

Validation Images

Figure 1: AIF1 staining on mouse spleen (red), counterstained with DAPI (blue).

Figure 2: AIF1 staining on mouse duodenum (red), counterstained with DAPI (blue).

Figure 3: Isotype control staining on mouse spleen (red), counterstained with DAPI (blue).

Figure 4: Secondary only staining on mouse spleen (red), counterstained with DAPI (blue).

Full Methods

Primary Antibody:

• Antibody: AIF1
• Catalog number: ABIN685477
• Lot number: 980993W

Secondary Antibody:

• Antibody: Alexa Fluor 546 Donkey Anti Rabbit
• Lot number: 1218269

Full Protocol:

• Frozen, OCT embedded tissues were cut 5 µm and mounted on positive charged slides.
• Sections were air dried for 30 min prior to fix in 10% neutral buffered formalin for 10 min.
• Fixed slides were rinsed three times in PBS for 5 min each at RT.
• Sections were blocked in 1 X PBS / 3% donkey serum / 0.1% Triton X-100 for 10 min at RT.
• Sections were incubated with primary antibody diluted 1:100 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) overnight at 4°C.
• Sections were rinsed three times in PBS for 5 min each at RT.
• Sections were incubated with secondary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 120 min at RT in dark.
• Sections were mounted on slides with FluoroGel II with DAPI (Electron Microscopy Sciences).
• Stained sections were imaged with a 3D Histech Pannoramic SCAN 150.

Experimental Notes: None

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Iba1 antibody (ABIN685477)

Ko, Shie, Lin, Chen, Yao, Chen: "Biodegradable Bisvinyl Sulfonemethyl-crosslinked Gelatin Conduit Promotes Regeneration after Peripheral Nerve Injury in Adult Rats." in: Scientific reports, Vol. 7, Issue 1, pp. 17489, (2018) (PubMed).

Hsu, Yao, Hsu, Lin, Chen, Chen: "Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model." in: Scientific reports, Vol. 7, pp. 42280, (2017) (PubMed).

Fu, Xia, Liu, Wang, Wang, Shi, Song, Song, Song et al.: "The acute exposure of tetrachloro-p-benzoquinone (a.k.a. chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin ..." in: Toxicology, Vol. 381, pp. 39-50, (2017) (PubMed).

Hu, Kodithuwakku, Zhou, Li, Han, Fang, Liu, Li: "Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway." in: Molecules (Basel, Switzerland), Vol. 22, Issue 6, (2017) (PubMed).

Furukawa, Nagaike, Ozaki: "Databases for technical aspects of immunohistochemistry." in: Journal of toxicologic pathology, Vol. 30, Issue 1, pp. 79-107, (2017) (PubMed).

Kouam, Yuan, Njayou, He, Tsayem, Oladejo, Song, Moundipa, Gao: "Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity." in: Frontiers in pharmacology, Vol. 8, pp. 653, (2017) (PubMed).

Ogawa, Onozato, Okuhara, Nagasawa, Tamura, Hayashi: "Spontaneous cutaneous soft tissue sarcoma with differentiation into fibroblasts in a Sprague-Dawley rat." in: Journal of toxicologic pathology, Vol. 29, Issue 2, pp. 119-24, (2016) (PubMed).

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background:

Synonyms: IBA1, IRT1, AIF-1, IRT-1, Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1, AIF1, G1

Background: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Gene ID: 199

UniProt: P55008

Pathways: Smooth Muscle Cell Migration