Iba1 antibody (AA 51-147) (Cy5)

Catalog No. ABIN685481

The Rabbit Polyclonal anti-Iba1 antibody has been validated for WB, FACS, IF (cc) and IF (p). It is suitable to detect Iba1 in samples from Human, Rat and Mouse. There is 1 publication available.

Quick Overview for Iba1 antibody (AA 51-147) (Cy5) (ABIN685481)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Iba1 antibody is conjugated to Cy5

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Iba1 Antibody (Cy5)

Binding Specificity: AA 51-147

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human AIF1

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Iba1 antibody (Cy5) (ABIN685481)

Song, Gaudin, King, Seo, Suh, Deng, Cui, Tietjen, Huttner, Saltzman: "Surface chemistry governs cellular tropism of nanoparticles in the brain." in: Nature communications, Vol. 8, pp. 15322, (2017) (PubMed).

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background:

Synonyms: IBA1, IRT1, AIF-1, IRT-1, Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1, AIF1, G1

Background: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Gene ID: 199

UniProt: P55008

Pathways: Smooth Muscle Cell Migration