SLUG antibody (AA 5-120)

Catalog No. ABIN685747

This Rabbit Polyclonal antibody specifically detects SLUG in WB, IF (cc), IF (p), ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Rat and Rabbit and has been mentioned in 3+ publications.

Quick Overview for SLUG antibody (AA 5-120) (ABIN685747)

Target: SLUG

Reactivity: Human, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLUG antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SLUG Antibody

Binding Specificity: AA 5-120

Cross-Reactivity: Human, Rabbit, Rat

Predicted Reactivity: Mouse,Dog,Cow,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Slug

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SLUG antibody (ABIN685747)

Bai, Chen, Wei, Li, Lin, Chen, Li, Du, Man, Zhang: "The zinc-finger transcriptional factor Slug transcriptionally downregulates ERα by recruiting lysine-specific demethylase 1 in human breast cancer." in: Oncogenesis, Vol. 6, Issue 5, pp. e330, (2017) (PubMed).

Kong, Guo, Liu, Zhao, Feng, Liu, Wang, Li: "Overexpression of SDF-1 activates the NF-κB pathway to induce epithelial to mesenchymal transition and cancer stem cell-like phenotypes of breast cancer cells." in: International journal of oncology, Vol. 48, Issue 3, pp. 1085-94, (2016) (PubMed).

Ge, Cao, Wang, Sun, Sun, Han, Cui, Liu: "Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-κB activation." in: OncoTargets and therapy, Vol. 9, pp. 1011-21, (2016) (PubMed).

Target Details for SLUG

Target: SLUG

Background:

Synonyms: SLUG, WS2D, SLUGH1, SNAIL2, Zinc finger protein SNAI2, Neural crest transcription factor Slug, Protein snail homolog 2, SNAI2, SLUGH

Background: Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis.

Gene ID: 6591

UniProt: O43623